Effects of cardiac contractility modulation on ventricular electrical remodeling in rabbits with chronic heart failure

AIM: To investigate the effects of cardiac contractility modulation (CCM) on ventricular electrical remodeling in a rabbit model of chronic heart failure. METHODS: The rabbits were divided into sham group, heart failure(HF) group and HF+ CCM group. The rabbit model of chronic heart failure was established by ligating the ascending aortic root. Then electrical stimulations during the absolute refractory period were delivered lasting 6 h everyday for 4 weeks in rabbits of HF+ CCM group. The QTc and ventricular effective refrective period (VERP) were recorded. The protein and mRNA levels of Kv1.4, Kv4.3 and connexin 43 (Cx43) were determined by Western blot and RT-qPCR. RESULTS: Compared with sham group, QTc were significantly prolonged in HF rabbits at week 12 (P<0.05). CCM therapy shortened QTc of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, VERP significantly increased in HF group and HF+ CCM group, while CCM therapy shortened VERP of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 were decreased in HF group and HF+ CCM group (P<0.05). However, CCM therapy restored the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 of rabbits with heart failure (P<0.05). CONCLUSION: CCM suppresses ventricular electrical remodeling in heart failure and the underlying mechanism may be associated with increasing Kv1.4, Kv4.3 and Cx43 expression.     

生态园林绿地建设中应用树木与草坪效果对比分析

本文从树木与草坪在用水量、观赏时间、造价、管理成本和环保上的 5项指标进行对比 ,论述了风沙、干旱地区的绿地建设应该是树木为本 ,花草点缀 ,乔木为主 ,灌木为辅的原则。     

丽水市竹业科技支撑建设

丽水竹产业实现了快速发展,其中科技起到重要的支撑作用.该文根据丽水市竹业技术现状和发展趋势,通过对竹产业的技术需求分析,提出了丽水市竹业科技支撑的主要内容、重点、方向和建议.     

苏云金芽胞杆菌Cry1Ba3 蛋白定点突变对杀小菜蛾活性的影响

Cry1Ba3是由本实验室发掘的对小菜蛾有高毒力的杀虫晶体蛋白。为了寻找对杀虫活性有重要影响的氨基酸,为杀虫机理研究和改造杀虫蛋白提供理论依据,本研究利用Ple Bio Informatique Lyonnais数据库对Cry1Ba3的二级结构进行模拟;采用BioEdit软件分析Cry1Ba3的疏水区;将Cry1Ba3与Cry1Aa1、Cry2Aa1、Cry3Aa1以及Cry4Ba1进行多序列比对,从而确定了20个氨基酸突变位点。利用半重叠引物PCR的方法对Cry1Ba3进行定点突变,将突变体在大肠杆菌BL21中进行诱导表达。通过浸叶法对各个突变蛋白杀小菜蛾的生物活性进行测定。获得的20个Cry1Ba3突变体均能在大肠杆菌BL21中表达,并以包涵体形式存在。杀虫活性测定结果表明M6(R192L)、M10(W303A)、M19(H485G)对小菜蛾的活性明显降低,二级结构预测表明活性降低的突变蛋白的构象均发生明显变化,其余17种突变蛋白的活性和二级结构都没有明显变化。说明第192位的精氨酸、第303位的色氨酸和第485位的组氨酸对Cry1Ba3的杀小菜蛾活性有重要影响,上述3个位点氨基酸突变引起的蛋白活性减低可能与毒素的空间结构变化有关。     

白玉兰离体培养和快速繁殖

试验建立了白玉兰离体繁殖体系。种子为外植体 ,初代培养基为 :B5 +BA0 .5mg/L +NAA0 .0 5mg/L + 30g/L糖 +琼脂 6g/L ,萌发率 85 %。继代培养基为 :B5 +BA1.5mg/L +NAA0 .15mg/L + 30g/glucose/sucrose + 6g/Lagar+Vco0 .5g/L ,月增殖率可达 5 .0± 0 .1。生根培养为 :1/2B5+NAA0 .5mg/L +AC1.0mg/L +VB2 0mg/L。以试管苗茎段、根段做外植体 ,培养基B5 +BA2mg/L+ 2 .4 -D0 .5mg/L +VC0 .5g/L ,可获得少量愈伤组织 ,继代存活率仅为 30 %。没有再分化成苗     

Analysis of the change in the original Metasequoia glyptostroboides population and its environment in Lichuan, Hubei from 1948 to 2003

Lichuan, located at the foot of the Wuling Mountain in southwest Hubei Province of central China, is well known in the world for the discovery of the living fossil Metasequoia glyptostroboides Hu & Cheng in the 1940s. Its natural habitat has been well protected by the Chinese government. In order to provide a scientific foundation for the protection of the Metasequoia resource, it has become necessary to analyze the changes in the original, natural Metasequoia mother trees (ONMMT) and their environment from 1948 to 2003. The results and countermeasures are as follows: First, the distribution areas have little changed, involving four towns/farmland, 45 villages, 5,746 individual trees in 1983 and 5,388 trees in 2003, covering nearly 600 km², with an average density of less than 0.1 tree/hm². The Metasequoia distribution consists of two main types: a scattered distribution type found mainly at the foothills or near houses, roads, villages and rivers while the population distribution type is found mainly at the mountains and valleys with a minority at low elevations in the mountains and basins. The largest Metasequoia populations have 105 and 123 trees. Second, in the past, attention was only paid to the protection of individual trees while the protection of the Metasequoia population and environment was neglected. This led to a shift from the past mixed forests to pure Metasequoia forests, with a simpler structure, reduced biodiversity and conditions non-conducive for natural renewal. From 1983 to 2003, 386 original Metasequoia mother trees died. So it is vital to enhance the protection of the only original Metasequoia population in the world and its habitat. Third, modern ecological means should be taken to protect the original Metasequoia population and its habitat. Research on the restoration and rehabilitation of Metasequoia vegetation should be carried out. Restoration and rehabilitation should be put into practice in the Xiaohe Valley, where the highest concentration of Metasequoia is found. Fourth, efficient measures should be taken to stop human activities that are harmful to Metasequoia and to improve the environment of ONMMT in order to promote the protection of the ONMMT population and its ecosystem. Eco-emigration and the return of marginal agricultural land to forestry can be practiced. __________ Translated from Acta Ecologica Sinica, 2005, 25(5): 972–977 [译自: 生态学报, 2005, 25(5): 972–977]     

葡萄利用结果枝副梢二次结果栽培试验

摘要：在桂林葡萄产区开展利用结果枝副梢促花二次结果的栽培试验，总结在新梢生长期喷多效唑（或矮壮素）、对新梢重度修剪促进冬芽提早萌发是整齐促放葡萄二茬果的关键技术，观测了二茬果果品品质和效益，提出了生产二茬果注意的技术问题。     

高含沙水流水轮机转轮的改型与抗磨蚀研究

进行高含沙水流水轮机转轮的改型研究，关键是要从正确确定转轮设计参数和轴面流道型式入手，根据三维水力设计理论，考虑导叶与转轮匹配关系进行叶型设计，针对不同破坏机理选择强磨蚀区采用金属喷涂或非金属保护等综合措施，使水轮机在高舍沙水流中运行时出力增加、抗磨蚀能力提高。     

对人工林非森林问题的探讨

人工林早已被人们所熟悉。在我国,有人工栽植树木,可追溯到1600年前;有成型的人工林时代,也有600年历史了。然而,世人热衷于人工林,主要还在于工业时代的到来,特别是近200年来。现在的人工林问题,已是全球关注的热点问题,这大致有两个原因:一个是环境问题,一个是利益问题。可是,至今为止,人工林非森林问题,并没有得到我们大家的接受,亦或我们的公众并不知道,人工林并不是森林。基于人工林问题的至关重要性,笔者认为,有必要通过讨论,达成共识,以便于对人工林的种种问题,有一个理性的、较早的觉醒。     

萝卜雄性不育小孢子发生的形态学研究

对萝卜雄性不育花进行形态学观察，发现两种类型的不育花，即花粉败育型和雄蕊萎缩型。比较研究了可育花及花种类型的不育花小孢子发育的细胞形态学特征，发现萝卜小孢子败育是个连续过程，在造孢细胞时期就已出现败育迹象，在小孢子发育过程中，绒毡层细胞出现液泡化、肥大、增生等异常现象。小孢子的败育与绒毡层细胞异常同步发生，小孢子一直信滞在单核期不再进一步发育，最终消失。另外，在正常可育小孢子发育过程中还偶尔观察到了不育花粉粒的存在。